This was pretty straight forward. I followed Dr. Frye's handy instructions and overnighted samples to him for analysis. I used a cotton swab to pickup about 6 samples and placed them on a moist paper towel in a ziplock baggie. I labeled them and sent them off. I received a call from Dr. Frye later the next day with the results:
- Red Galacts - high load of hook worm
- Lorenzo - moderate hook worm
- Bakhuis - moderate hook worm
This was more interesting for me because it was the first time I ran these tests. I exchanged email with Ernie Colaizzi at Research Associates Laboratory (vetdna.com). He was very helpful walking me through the collection process using tools I could find in local stores. His recommendation was to use foam tip makeup applicators to collect samples instead of cotton swabs. He said the foam releases DNA better and allows for detection of low level infections.
I picked up gloves and applicators:
I collected samples on Sunday night. I did not get pictures of the process since it was only me and there were 12 jumpy frogs bouncing around that required two handles to wrangle. I managed to swab all four frogs within each group. I swabbed their backs, and I managed to swab the legs and bellies of most (but not all). I used one swab per group. After swabbing I sealed the foam applicator in a small ziplock bag, filled out the online form (one for each swab) and then stapled the ziplock back to each form.
I mailed them out on a Monday morning. Results were back Wednesday afternoon - negative all the way around!
The blacked out sections are my account number and address. The total cost was $120 plus about $3 for shipping an envelope. The fee breakdown was $20 for Chytrid and $20 for Ranavirus for a total of $40 per group. With three groups, my total was $120.
That was my experience with the testing. It's pricey but it was well worth the piece of mind. So per group, I'd break down the costs for testing as:
- $18 fecal
- $20 overnight USPS
- $20 Chytrid
- $20 Ranavirus
- $3 USPS mail / large envelope
- $81 estimate for a single round
I did reduce the shipping costs by testing three groups at once, but that gives you a rough idea of the outlay for a first round of tests. I found the process interesting and thought I'd share my experiences with the process. Now on to dealing with those hooks!